On days 3 and 5, 25 l of medium was added to the drops and, after pipetting and rinsing the embryos, the same amount was withdrawn without disturbing the embryos.
After rinsing to neutrality, samples were gently stir red and decanted several times in order to remove all organic matter from the remaining mineral fraction, which was then discarded.
The cleaning regime generally involves a manual cleaning stage using a brush, to remove all organic matter and other debris, followed by disinfection and finally rinsing with clean water.
Rinsing was repeated, and excess 2% w/v sodium chloride solution was added to the seeds with agitation for 20 min, followed by a final rinse with sterile distilled water.
Vacuum pressure was maintained at 0.7 bar during filtering and rinsing.
Sixty ml of water was used for rinsing the sample bag and the water was then mixed with the herbage.
The composition of rinsing medium, calibration samples and reagents were adjusted to those of the digest solutions.
After rinsing to neutrality, another acid wash followed; subsequently another rinsing to neutrality was performed.
