After 5 min fish were removed, rinsed briefly in carbon-filtered water, and returned to aquaria.
Ten to twenty grains were selected from each sample, washed with methanol in an ultrasonic bath and rinsed with distilled water.
The lungs were removed, rinsed twice, placed in a universal tube and minced with sharp scissors to small pieces (<0 - 5 mm3).
The floating inflorescence remainders were decanted off, and the seeds were rinsed in distilled water.
The skins were rinsed in saline solution and patted dry : the surface lipids were left intact.
The gills were removed and rinsed, and the arches were examined individually.
Below the aortic valve, on the left ventricular surface, a two centimeter-long vegetation was debrided and the left: ventricle and aorta rinsed with iodine solution.
The incubation bottles were then removed, and the samples rinsed several times with washes of ' cold ' lagoon water.
