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After rinsing with fresh ether, the abdomen was again dried for 3 days and weighed again.
The 48 stalls kept water in buckets for rinsing hands and tableware, reusing the same water several times during the day.
After several centrifugation steps for rinsing, pieces are triturated with a flame-polished pipette, pelleted, and then resuspended for plating.
Any residual shards of sarcotesta were removed by rinsing in distilled water, and the surface of the clean seeds was blotted dry.
The seeds were first surface-disinfected by immersing in 95% ethanol for 30 s and then rinsing five times in sterile distilled water.
All collecting implements were thoroughly scrubbed between each sample using fresh water and a mild detergent before twice rinsing in distilled water.
The tubes and residue on the filter paper were rinsed twice with 5 ml deionized water and the rinsings added to the extract.
After rinsing to neutrality, another acid wash followed; subsequently another rinsing to neutrality was performed.
The composition of rinsing medium, calibration samples and reagents were adjusted to those of the digest solutions.
Sixty ml of water was used for rinsing the sample bag and the water was then mixed with the herbage.
Vacuum pressure was maintained at 0.7 bar during filtering and rinsing.
Rinsing was repeated, and excess 2% w/v sodium chloride solution was added to the seeds with agitation for 20 min, followed by a final rinse with sterile distilled water.
The cleaning regime generally involves a manual cleaning stage using a brush, to remove all organic matter and other debris, followed by disinfection and finally rinsing with clean water.
After rinsing to neutrality, samples were gently stir red and decanted several times in order to remove all organic matter from the remaining mineral fraction, which was then discarded.
On days 3 and 5, 25 l of medium was added to the drops and, after pipetting and rinsing the embryos, the same amount was withdrawn without disturbing the embryos.
