The conjunctiva, sclera and iris are heavily pigmented with melanin granules and the iris contains gold-reflecting material, which is mostly obscured by the pigmented layer.
A motorized micromanipulator was used to position a 30-gauge needle against the sclera, about 2 mm posterior to the limbus.
It is possible that different signals modulate growth of the choroid and sclera.
The retina was carefully removed from the choroid and sclera during the initial stages of fixation.
The retina was removed from the sclera and retinal pigment epithelium, trimmed, and cut into two pieces.
Nitric oxide synthase-containing cells in the retina, pigmented epithelium, choroid, and sclera of the chick eye.
The sclera and pigment epithelium were then gently dissected off of the retina.
The sclera and pigment epithelium were removed and the retina flattened, photoreceptor layer uppermost, on a gelatinized slide.
