The oocytes were then denuded completely by pipetting, mounted on slides, fixed and stained as described above, and examined under the differential interference microscope.
The cumulusenclosed oocytes were denuded by repeated pipetting to release the cumulus cells.
In the present experiments we used denuded oocytes from which the egg envelopes (jelly coat and vitelline coat) had been removed.
In vitro fertilization of tubal rabbit ova partially or totally denuded of follicular cells.
After the maturation culture, the oocytes enclosed by cumulus cells were completely denuded by pipetting.
The accompanying illustrations must therefore be considered as representing these structures in a partly denuded condition.
Oocytes cultured for maturation were partially denuded by gentle pipetting in a maturation medium containing 0.1% (w/v) hyaluronidase.
After the maturation culture, the oocytes were denuded, stained, and examined as described above.
