For each combination of temperature and salinity ("treatment"), there were three replicate aquaria.
Infection time was 30 min during which the water was continuously aerated using aquaria pumps.
By then the glass-walled aquaria may seem but dowdy relics of a passing fashion of the sixties.
Fish were transferred gently to large covered glass aquaria provided with a continuous flow of fresh seawater and fed fresh chopped fish every 2 days.
Fish were immediately returned to aquaria where the infection was allowed to cycle.
Since the fish were caught on different days, they were also transferred to the experimental aquaria on different days.
Fluorescent lights were positioned in each unit to provide lighting intensities similar to those observed at the sides of the aquaria in the zebrafish module.
The mean parasite load per recipient were approximately equal in the six infection aquaria, ranging from 12 to 18.
