These spheroids were mechanically dissociated and re-cultured as free floating secondary spheres to generate further spheroid colonies.
It has a uniform thickness and a prominent, dense layer derived from spheroids formerly present in cortical granules.
To make our investigation more comprehensive, we examine cells with a variety of unstressed shapes including oblate and prolate spheroids.
The irregular behaviour around the tip of the spheroid is indicative of membrane folding.
As noted above, the latter surfaces bulge out slightly near the equator, relative to the rotating spheroid.
However, viscous stresses generated internal to the globule stretch the occluded droplet into a rather extended prolate spheroid.
Such models accurately reproduce the growth of spheroids and the macroscopic heterogeneity which is the hallmark of spheroids.
The tip of the spheroid is indicated with a dot.
