To keep seed availability roughly constant throughout our experiments, baits were replenished when necessary.
This is not ideal for long-term experiments because the chamber must be replenished with air periodically, subjecting the seeds to abrupt changes in oxygen concentration.
Both the honey and the cotton sticks were replenished at weekly intervals and care was taken to keep cotton sticks wet.
The leakage there was made as small as possible and was replenished by continually supplying air to the inside of the model.
The salt solutions in the desiccators were replenished as needed to maintain fully saturated conditions as water was absorbed from the seeds.
Medium was left unchanged for the first 6 days and replenished every 3 days.
Mortality was recorded daily and the honey replenished as needed.
The culture medium was replenished every other day with freshly prepared medium.
