The dotted line represents the part at the part mating frame in the robot workspace.
Females were replaced every 4 days for six sequential matings in the first experiment and nine sequential matings in the second experiment.
Homozygous mutants with only 2 % residual enzyme activity were recovered in progeny of inter se matings of heterozygotes.
We wanted to maximize the number of second matings and minimize possible multiple mating with the second male.
It may be possible that this variation is associated with differences in mating success among males.
In other words, mating might be less costly in outcrossing than in selfing species.
If the weather is cool, then mating, and subsequent oviposition, are delayed.
Unreceptive females either flew away or prevented mating by a series of aggressive movements.
