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The membranes used in a feeding device can therefore be prepared in advance, frozen and only thawed as needed.
We first froze mouse mature testicular tissue, then we injected the thawed spermatogenic cells into oocytes.
Samples were thawed slowly and cut up for examination.
Aliquots of the blood are thawed and used as required.
Thawed chops were removed from the bag and the loin muscle removed from the bone.
A fresh stock of virus was thawed immediately prior to each injection.
Before use, they were thawed, added to the medium and subjected to sonication.
Using phase contrast microscopy, control slices showed more cell outgrowth compared to thawed slices.
Immediately prior to being assayed, thawed saliva samples were centrifuged at 14,000 rpm for 7 min to remove particulate matter.
On the day of use, an aliquot was thawed and diluted in the culture medium to the desired working concentrations.
To ensure infectivity, the prepared vaccine must be used within 8 hours of thawing and, once thawed, should not be refrozen.
About 7 days before each individual test, worms were thawed and grown for at least 2 generations.
On the day of the assay, the samples were thawed and frozen quickly three times in liquid nitrogen to rupture cell membranes.
