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Following a pressure increase in the pipette, the cell suspension was transferred to seminiferous tubules (dark blue).
The target is picked up by the mechanical micromanipulator with the pipette quite easily.
There is no research work on the touch sensor which can be set easily at the tip of the pipette.
A feeding apparatus, composed of a membrane, reservoir and blood was fitted on the pipette during bloodmeals.
The injection pipette was then inserted through the zona pellucida into the middle of the oocyte.
They were applied equidistant from control and the pipette tip containing the volatile chemical immediately after the application of the chemical being tested.
This substance was removed with the aid of a pipette and placed on formvar- and carbon-coated grids.
Eggs were placed in fresh pipettes in batches of approximately 30, where a new cycle could begin.
In fact, spermatozoa could have been expelled from the ooplasmic furrow created by the injection pipette.
The injection pipette was withdrawn and the oocyte released from the holding pipette.
At the end of each experiment, seal resistance was assessed by withdrawing the patch pipette to obtain an outside-out patch.
A thirteenth flush line, active between applications, ensured the removal of any previous drug left in the pipette tip before a new application.
Those unable to right themselves after probing with a pipette tip were recorded as 'knocked-down' and excluded from the final analysis.
The upper panel (a) shows a glass fiber in contact with a small, functionalized bead supported on a movable pipette.
The donor cell was then drawn in and out of the injection pipette until the cell membrane was broken.
