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After culture, some oocytes enclosed by cumulus cells were completely denuded by pipetting.
Pretreatment of the denuded segments with indomethacin also reduces their sensitivity to thromboxane.
The cellular bodies may have originated from denuded areas of cytoplasm which had budded off from the mis-shapen enterocytes.
Denuded oocytes were matured in vitro, spontaneously or by progesterone treatment (1 ug/ml), and inseminated with homologous spermatozoa.
Incompetent oocytes were denuded and preincubated in the presence of the blockers for 30 min and maturation was then induced by progesterone (1 g/ml).
What little 'trickle-down' there was trickled mainly into provincial capitals, and local people themselves were often left with little but denuded hillsides and poisoned rivers.
The oocytes were denuded of cumulus cells by repeated pipetting.
Hence, they were denuded for a short time between recovery and fertilisation.
By this time, the ridges along the lake would have been completely reoccupied and denuded of large stands of forest.
Environmental interventions such as soil conservation, afforestation, and rehabilitation would be adopted to restore denuded areas.
Oocytes with compact cumulus layers and semicircular cytoplasm, classified as degenerated, were discarded, as were oocytes with expanded cumulus layers and denuded oocytes.
Relative to the fertilised oocytes, 61.0% and 39.4% of cumulus-intact and denuded oocytes were monospermic.
Oocytes were then denuded completely by pipetting with a small-bore pipette.
As a result, responsive to norepinephrine than arteries from denuded arteries from pregnant animals are more nonpregnant animals.
The denuded oocytes were then transferred into 500 l of fresh development medium.