Exempel på biotin

• Biotin is involved in the basic metabolism of all organisms.

• This enzyme deficiency is found in about 1 in 60,000 newborns and can be treated with daily doses of biotin.

• Other important nutrients include vitamins D, C, B6, K and biotin (found in liver, wholegrains, eggs, fish and nuts).

• Gelatine and biotin help promote healthy nail growth by acting at the base of the nail where nail growth begins.

• Biotin may even stimulate new hair growth if the hair loss can be traced to a biotin deficiency.

• To make such particles, solutions containing streptavidin coated polystyrene particles and biotin coated gold nanoparticles (1-5 nm diameter) are mixed.

• The different, labeled amino acids in the figure all contribute to the total biotin binding energy.

• This is denatured and hybridized with a biotin-labelled oligonucleotide 'capture probe' designed to anneal to a specific internal sequence of the fragment in question.

• The reaction was revealed with biotin-avidin peroxidase system.

• Sensing discrete streptavidin-biotin interactions with atomic force microscopy.

• Once the particles are trapped, streptavidin is introduced in solution at the trap location and bond with the biotin on the particles.

• It is to these moieties (biotin-steptavidin) that signal-generating systems attach to.

• It is assumed that much smaller biotin-derivatized molecules can easily collect together at this density.

• In this system, the 5h-end of an oligonucleotide linked to biotin was used as the primer in the sequencing reaction.

• The dark stained parts indicate the biotin-streptavidin complex.

• Recently a number of biotin derivatives have become commercially available, and they have various different binding characteristics.

• The profiles revealed that the individual spots bound to one or both biotin derivatives.

• In this case the limiting factor is the low surface density of the biotin ligands.

• In contrast, quantities of high molecular weight, biotin-labelled surface proteins increased with greater centrifugal force, indicating labelling of otherwise inaccessible residues.

• Before use, the labeled probes were precipitated with ethanol, then washed to remove unincorporated biotin-labeled nucleotides.