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The peripheral asters come into close apposition with the cortex.
Indeed, the antibody stained the asters and (more strongly) the vesicular components attaching to the periphery of the mitotic apparatus.
Although the paternal centrosome duplicated, the maternal centrosome in the centre of the egg aster did not divide.
The microtubules of the male aster are arranged in a funnel-shaped array, all stretching to the fertilisation area in the cortex.
Migration of the asters sets one in the centre of the egg and the other in the cortex.
Microtubules of both asters reaching into the cortex cross each other cirumferentially.
This indicates that neither aster is strongly attached to the cortex until the end of metaphase.
The lower ('vegetal') spindle pole produces a much larger aster than the upper ('animal') spindle pole.
Again, the microtubules of the central aster run along the pronuclear surface and reach the cell cortex.
All microtubules associate with the meiotic spindle and asters.
However, fully developed microtubule asters were not observed.
These asters grow until the male pronucleus reaches its female counterpart and disintegrate afterwards.
A small microtubule aster was observed in association with decondensed chromatin following nuclear transfer, suggesting the introduction of a somatic cell centrosome.
In early metaphase, a bipolar spindle with broad poles and devoid of asters is formed.
At metaphase the two equal-sized asters span the entire egg in a symmetrical arrangement.