All are oriented with the anterior to the right and show the mouthparts, the two dorsal branches of the tracheae and the midgut.
From that point on, the embryos that did not hatch progressed through the stages of brown midgut, brown tracheae and death.
However, we were unable to dissect the tracheae without damaging them, so we cannot exclude dissection damage as the cause of this staining.
Both genotypes therefore display the same progression of phenotypes, from brown midgut to brown tracheae to death.
In this way the host's tracheae come to lie along the wall of the trophamnion.
By about 50 hours after fertilization, all the embryos had both brown tracheae and midgut, and were starting to turn completely brown.
This network of transverse and longitudinal tracheae equalizes pressure throughout the system.
These membranes vibrate rapidly, and enlarged chambers derived from the tracheae make the cicada's body serve as a resonance chamber, greatly amplifying the sound.