The digital data from the glasshouse experiment gave improved discrimination of treatment effects compared with the visual-rating data.
In glasshouse tests, all six cultivars were intolerant of feeding by cowpea aphid.
The trays were kept well watered in an unheated glasshouse for a year.
Each sheaf was then stored in a dry, unheated glasshouse pending sampling of intact spikes for sprouting tests.
However, laboratory studies can overestimate the level of control achieved by biocontrol agents in the glasshouse.
Seven-day-old seedlings were transplanted into 10 cm diameter clay pots, at four seedlings per pot, and placed in metal trays outside the glasshouse.
Prior to laboratory or glasshouse experiments, seeds were scarified and germinated in the dark on moist filter paper for two days at room temperature.
Such a highly resistant clone could most easily exist from season to season in a glasshouse environment.
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