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Coltiviruses have been isolated from several mammalian species (including humans) and from ticks and also mosquitoes.
Infected mosquitoes can also travel in ships and trains and spread the disease.
The presence of suitable containers in and around households increases the risk of oviposition and the production of immatures and adult mosquitoes.
They showed that gametocyte counts in the mosquitoes are, like macroparasite intakes, heterogeneous (overdispersed).
Also, if mosquitoes had been a major vector, the pattern of spread would have been expected to be random across the sites.
Most mosquitoes were aggregated around the rear of the flight section.
Mosquitoes were allowed to feed immediately on this mouse.
Our study highlights one type of environmental stress in which resistant, genetically manipulated mosquitoes may have to compete with natural vector populations.
It is evident that many mosquitoes were able to retain virus for a period up to 6 days post feeding on an infected animal.
Specifically we investigate levels of heterozygote deficit in the genotypes of oocysts from multiply infected mosquitoes.
Female mosquitoes were introduced into a holding tube from which they could escape into a cage through a one-way funnel.
Spatial clustering indicates that more mosquitoes were found in some houses than others.
The total number of mosquitoes that were collected on a particular day was therefore pooled for analysis purposes.
Transmission of virus was then attempted by allowing the potentially infected mosquitoes to feed on six susceptible cattle at various times post-infective feeding.