Of course, it is possible that more complex patterns of gene regulation in eukaryotes might predispose them to synergistic effects between mutations.
Mutations that lead out of the valley onto a new fitness peak are, of course, beneficial mutations.
In the present study we made additional mutations.
The molecular characterization of the mutations suggests a low frequency of false positives.
Heritable changes- called" mutations" - occur if the genetic message is changed.
Any divergence in genotypes or phenotypes is due to the recent generation and fixation of new mutations in these two separately maintained lines.
All data related to mutations are presented with reference to these sequences.
Two control samples were included in each experiment to normalize for mutations induced by transfection or superinfection processes.