0 a type of paint that you put on a surface before the main paint is put on: --
1 a type of make-up that you put on your face under other make-up to make it go on more smoothly and last longer: --
2 a small book containing basic facts about a subject, used especially when you are beginning to learn about that subject --
3 a talk, experience, etc. that provides information from which you can learn: --
I think I should give you a quick primer on the basic setup and cast of characters.
5 a type of paint put on a surface before putting on the main paint --
The first sequencing reaction is performed using the primers that hybridize to the vector sequence and polymerize the strand complementary to the template.
With the obtained sequence data new primers were designed to check whether the results could be repeated.
Multiple sequencing primers were used to obtain complete sequence of the 1600-bp fragment with each clone having, on average, 5-fold sequence coverage.
Underlined nucleotides mark the position of the primers.
The primers were based on the central conserved region and the 5h region of the respective genes [14, 15].
The result obtained with primers 2 and 4 is presented in panels (a) and (b), respectively.
With appropriate primers the method can also be adapted for the recovery from soil of geohelminth eggs of other parasite species.
The gene coding for the elastase was amplified using primers specific for the sequence of cercarial elastase and was cloned into a mammalian expression vector.
