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Growth of the two layers of the chick sclera is modulated reciprocally by visual conditions.
The retina, choroid, and pigment epithelium were dissected as a unit from the sclera.
The evidence for visually guided regulation of eye growth is beyond refute, the choroid and sclera being the two principal targets.
Rather, the local anesthetic lidocaine was topically applied to the sclera prior to injection.
Suction was used to remove the vitreous; the sclera and retinal pigment epithelium were peeled away.
The upper eyelid, the nictitating membrane, and the sclera were excised.
Retinas were isolated by dissecting the eyes from their orbits, then lightly brushing the retina apar t from the sclera and pigment epithelium.
The sclera and pigment epithelium were removed and the retina flattened, photoreceptor layer uppermost, on a gelatinized slide.
The sclera and pigment epithelium were then gently dissected off of the retina.
Nitric oxide synthase-containing cells in the retina, pigmented epithelium, choroid, and sclera of the chick eye.
The retina was removed from the sclera and retinal pigment epithelium, trimmed, and cut into two pieces.
The retina was carefully removed from the choroid and sclera during the initial stages of fixation.
It is possible that different signals modulate growth of the choroid and sclera.
A motorized micromanipulator was used to position a 30-gauge needle against the sclera, about 2 mm posterior to the limbus.
The conjunctiva, sclera and iris are heavily pigmented with melanin granules and the iris contains gold-reflecting material, which is mostly obscured by the pigmented layer.
