Voorbeelden van inseminated

• The stages at which human fertilization arrests: microtubule and chromosome configurations in inseminated oocytes which failed to complete fertilization and development in humans.

• After culture, some oocytes (onethird) were fixed to examine nuclear maturation; subgroups of oocytes were inseminated or used for glutathione assay.

• Cumulus-oocyte complexes were collected from slaughterhouse ovaries, matured, inseminated and subjected to embryo culture in vitro.

• Our results indicate that with this improved in vitro system, more than 50% of inseminated oocytes can develop to blastocyst stage.

• In a series of experiments, empty zonae were inseminated, then washed thoroughly to remove all spermatozoa from the outer zona surfaces.

• In the control group, in contrast, spermatozoa penetrated 29 (36.3%) of 80 diploid embryos inseminated for the first time at the 2-cell stage.

• Oocytes were inseminated with spermatozoa that had undergone the spontaneous acrosome reaction in normal medium.

• Inseminated females producing no larvae over this 6-day period were considered sterile.

• Oocytes were inseminated with 5 x 104 motile spermatozoa and cultured under the same conditions.

• The final purpose of this study was to investigate the possibility of using this activation method to activate inseminated oocytes that were not fully activated.

• Oocytes inseminated after the first minute of activation did not become fertilised, indicating that the block to polyspermy had been established.

• Only ten out of the 313 females that mated were not inseminated and seven of these mated with irradiated males.

• All eggs remained unactivated until they were inseminated by the conventional method.

• In the second experiment, 380 ewes were inseminated at different times before or after ovulation, producing 537 lambs.

• The other inseminated oocytes were further cultured for up to 8 days.