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For each of the three treatments (undiluted, 10x2 and 10x3) two rabbits were inoculated by intramuscular inoculation.
Incubation vessels inside the chamber were filled with sterile sand, inoculated with crushed sandstone-based biofilms and allowed to incubate for 5.5 months.
Three control cultures, consisting of sand-filled vessels that were not inoculated, were included in the incubation chamber.
Animals were inoculated with viable metacercariae in water.
Within any 1 trial, 4 voles of each treatment were inoculated by random turn from the same infected donor vole.
The control mice were inoculated per os with 500 larvae/animal using a 2 ml syringe connected to a gavage needle.
To examine microbial responses, we inoculated these solutions and wet carbonaceous chondrite meteorites with microorganisms.
All birds were reared in a coccidia-free environment until inoculated or otherwise used.
The cortisol levels of the control eels which were not inoculated with larvae did not show any significant change over the experimental period.
At this time they were divided into separate cages according to genetic line and were immediately inoculated orally.
No clinical signs or seroconversion were observed in the uninoculated sentinel rabbit kept in the same room as the inoculated rabbits.
To maintain a constant high level of immune resistance, young stock must be regularly inoculated.
After centrifugation at 3000 rpm for 15 min, 75 ml of the supernatant was inoculated on to two wells of each cell line.
The plates were then inoculated with respective phages using a multipoint inoculator.
Two groups of four rabbits were then inoculated with inactivated virus via oral and intramuscular routes.
