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At no time did parasitoids physically touch the central filter paper discs that had received no treatment or treatment with hexane alone.
The waxy layer of the vitelline membrane was then removed by sequential treatment with isopropanol and hexane.
The control stimulus was a similar pipette containing a filter paper impregnated with a 10-ml aliquot of hexane.
Control treatments with 1 ml droplets of hexane alone did not stimulate aphid movement.
The hexane extract was removed for column chromatography taking extreme care not to damage the mosquito sample.
Samples were desorbed with 5 ml of hexane.
This was diluted with hexane for easier application; the hexane soon evaporated.
The hexane was evaporated and the oil weighed.
Traps containing septa impregnated with hexane caught significantly fewer males of both species (fig. 4d).
The required amounts and ratios of synthetic compounds dissolved in 200 l hexane were applied onto the inner surface of the rubber septa.
Significantly fewer males were caught in control traps impregnated with hexane only.
After the 24-h collections, volatiles were eluted from the traps with 1 ml hexane (99.5%).
Another vial contained 0.5 ml hexane, but no eggs.
