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To encourage oviposition, filter paper was placed on top of moistened cotton wool in a tubular support at the base of the cages.
A small container of cotton wool soaked with water was also placed in the experimental arena, as were pollen, honey and sugar cubes.
All air entering the building passed through cotton wool filters.
A small container of cotton wool soaked with water was also placed in the experimental arena so flies were permanently water-sated.
A piece of cotton wool saturated with 10% honey solution was supplied in each vial as food source for the adult wasps.
The cut stems were placed in a conical flask filled with water and sealed around the base of the stem using cotton wool and parafilm.
All cocoons that formed from parasitized hosts were removed from the stems and kept separately in vials plugged with cotton wool.
Leaves were maintained fresh during the experiment by small plastic vials with water and cotton wool surrounding the petiole.
The tubes were covered with cotton wool and kept in an incubator at the temperature and relative humidity indicated above.
Each vial contained a single wasp and the vial was closed with two plugs of cotton wool before release.
Each assay was performed with two germination chambers (replicates) containing 50 seeds placed on a layer of cotton wool moistened with distilled water.
Both ends of each tube were plugged with cotton wool.
Emerging insects were provided with water-soaked cotton wool, honey droplets, and a yeast/sugar diet.
In order to release the parasitoid, each vial could be opened from the outside of the container by pulling strings that were attached to the cotton wool plugs.
An 8% sugar-water solution put in a vial plugged with cotton wool was provided to feed the moths, and balsam fir branches bearing lichens were added for oviposition.
