The structure was consistent with a phosphatase, and this was confirmed by trying a variety of potential substrates.
A specific protein tyrosine phosphatase can deactivate the kinase.
Cytoskeletal integrity in interphase cells requires protein phosphatase activity.
In addition, the enzyme was not inhibited by levamisole and ammonium molybdate, excluding alkaline phosphatase and nucleotidase activities, respectively.
Net protein phosphorylation represents both in vitro protein kinase activities and dephosphorylation by protein phosphatases during the assay.
The ineffectiveness of alternative protein phosphatase inhibitors to stimulate germination supports this possibility, but additional viability assays in the presence of these compounds are necessary.
Immunoreactivity was revealed with goat anti-mouse alkaline phosphatase conjugated antibody and dot-blot immunoreactivity was expressed in arbitrary, relative optical density units.
Pascual et al.46 showed that total and immobilized urease and phosphatase persisted in soils for 360 days after compost application.
