The fixative and sucrose are able to diffuse through the foam, cryoprotecting and postfixing the specimen.
Although a high glutaraldehyde fixative might have maintained this attachment, glutaraldehyde was incompatible with the immunohistochemical studies performed on these eyes.
The fixative was spread by continuous and gentle blowing until the cytoplasm disappeared.
The eyeballs and brains were removed and postfixed for 1 h in the same fixative.
After 24 h the fixative was renewed and the nodules were stored in the same fluid prior to sectioning.
In addition, many fixatives chemically alter the fixed material to make it less palatable (either indigestible or toxic) to opportunistic microorganisms.
The sclera was sliced open to admit fixative and in a manner to define the orientation of the eye.
Neonatal kittens were also per fused intracardially with the same fixative using the same procedures except that they were not artificially respirated.
