This indicates that neither aster is strongly attached to the cortex until the end of metaphase.
Microtubules of both asters reaching into the cortex cross each other cirumferentially.
Migration of the asters sets one in the centre of the egg and the other in the cortex.
Indeed, the antibody stained the asters and (more strongly) the vesicular components attaching to the periphery of the mitotic apparatus.
A simplified technique for the observation of asters in amphibian eggs stratified by centrifugation.
In early metaphase, a bipolar spindle with broad poles and devoid of asters is formed.
However, fully developed microtubule asters were not observed.
The female pronucleus formed during this time and was located at the leading edge of the egg aster.
