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After thawing, the samples were assayed in duplicate analyses using a standard time-resolved fluorescence immunoassay.
Aliquots of the blood are thawed and used as required.
In this study, the tolerance of algae and cyanobacteria to freezing, thawing and freezing-drying stresses was evaluated by short-term (days to months) low-temperature experiments.
Samples were thawed slowly and cut up for examination.
The column was subjected to several cycles of top-down freezing and thawing to simulate an active layer.
The membranes used in a feeding device can therefore be prepared in advance, frozen and only thawed as needed.
A fresh stock of virus was thawed immediately prior to each injection.
Before use, they were thawed, added to the medium and subjected to sonication.
On thawing cells don't regain their original shape and turgidity.
In contrast, summer represents warmth and life in the second stanza, as it thaws the ice.
We first froze mouse mature testicular tissue, then we injected the thawed spermatogenic cells into oocytes.
In preparation for assay, the samples were rapidly thawed and centrifuged for 10 min to remove coagulated protein and other insoluble material.
Immediately prior to being assayed, thawed saliva samples were centrifuged at 14,000 rpm for 7 min to remove particulate matter.
On the day before testing the thawed specimens were stripped carefully all the soft tissues leaving the ligaments and joint capsules intact.
Successful freezing of unfertilized mouse oocytes and effect of cocultures in oviducts on development of in vitro fertilized embryos after thawing.
