Only those oocytes surrounded with a multiple-layered compact cumulus oophorus were used.
Injected cumulus cell was enlarged and its mitochondria remained associated closely with the nucleus.
After culture, some oocytes enclosed by cumulus cells were completely denuded by pipetting.
The oocytes surrounded by compact cumulus and with evenly granulated cytoplasm were selected.
Only oocytes with evenly granulated cytoplasm surrounded by multiple layers of compact cumulus cells were used in all the experiments.
Only oocytes with one or more complete layers of unexpanded cumulus cells and an evenly granulated cytoplasm were selected.
Only oocytes with one or more completed layers of unexpanded cumulus cells and evenly granulated cytoplasm were used.
Factors influencing resumption of meiotic maturation and cumulus expansion of porcine oocyte-cumulus cell complexes in vitro.
