Mates were marked with nail polish and reared in the conditions described above.
The retina was mounted in 100% glycerol under a raised coverslip, which was sealed with clear nail polish to prevent dehydration.
After the gel had hardened again, the sides of the cover slip were covered with transparent nail polish to prevent infection by fungi.
Last, the preparation was coverslipped and sealed with nail polish to prevent dehydration.
The coverslip was mounted on a slide sealed with nail polish.
Snails were examined 7 weeks after miracidial exposure, and each snail that released cercariae was marked with nail polish and placed in new cultures, 10 per culture.
The slides were sealed with nail polish.
The slide was sealed with nail polish.
