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• The digital data from the glasshouse experiment gave improved discrimination of treatment effects compared with the visual-rating data.

• In glasshouse tests, all six cultivars were intolerant of feeding by cowpea aphid.

• The trays were kept well watered in an unheated glasshouse for a year.

• Each sheaf was then stored in a dry, unheated glasshouse pending sampling of intact spikes for sprouting tests.

• However, laboratory studies can overestimate the level of control achieved by biocontrol agents in the glasshouse.

• Seven-day-old seedlings were transplanted into 10 cm diameter clay pots, at four seedlings per pot, and placed in metal trays outside the glasshouse.

• Prior to laboratory or glasshouse experiments, seeds were scarified and germinated in the dark on moist filter paper for two days at room temperature.

• Such a highly resistant clone could most easily exist from season to season in a glasshouse environment.