For the standard method, half the value of the minimal screening dilution used was assumed to be an exact value.
Then 100 ml of serum samples were added at a dilution of 1 : 100.
The infinite dilution thermochemical properties of compounds are used if data is available and predicted with group contribution methods when none exists.
Results were expressed as dilutions, with titres equal to or higher than 1:32 regarded as positive.
Aqueous dilutions of these samples were exposed to the cercariae through membrane filters.
This approach requires numerous dilution series to ensure synchronisation between the linear range of amplification of the unknowns and the competitors.
The incubation of both primary and secondary antibodies (1:500 dilution for both) was carried out in humidity chamber.
Thus, these curves can be used to extrapolate the scattering to infinite dilution.
