For two compounds (glycerol and glyceric acid) abundances were determined on the same order of magnitude as for the amino acid glycine.
This protein spot was used for amino acid sequence analysis.
The most frequent amino acid whose sidechain is contacting the magnesium ion is aspartate (42 %), followed by threonine (23 %), glutamate (17 %) and serine (10 %).
Statistical coupling is the extent to which an amino acid at one site changes in response to a perturbation at another site.
Environment affects amino acid preferences for secondary structure.
The affected protein may be misfolded or truncated, or it may contain an expanded repeat or amino acid substitution.
Therefore, the delicate folding process of many membrane proteins makes them particularly sensitive to single amino acid changes.
One subunit (top right) has been displaced to display the location of the amino acid substitutions (in cyan and white).