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Maximum values obtained from measurements vary in liver flukes from the three host species (table 2).
Human infections with the lung fluke are acquired by eating raw or undercooked freshwater crabs containing live metacercariae.
This temperature has also been used for storing metacercariae by other authors, thus enabling comparative analysis with lowland liver fluke isolates.
We then focus on flukes and snails, highlighting important biological traits with regard to population structure.
Naturally infected flukes were identical with those recovered from experimentally infected fish.
In the second group of six rabbits each given 200 metacercariae, five were infected, with two or three flukes per host.
When metacercariae harvested from the snakes and intercostal muscles of rodents were infected orally to cats, they developed into adult flukes in the small intestine.
Muscle fibres enzymatically isolated from the fluke have been characterised in terms of their morphology and contractile proteins.
Isolated muscle fibres from the ventral sucker may represent a more homogeneous population of muscle fibres than fibres isolated from a whole fluke digest.
The main cause of anaemia in fascioliasis is the passage of red blood cells into the gastrointestinal tract, presumably via flukes and the bile duct.
Following entry into the liver, the fluke burrows through the liver parenchyma before reaching its final site in the bile duct.
In contrast, follicles were present in flukes at the earliest time of recovery from the bile duct, namely, 5 weeks 3 days post-infection.
Partially digested ventral suckers of adult flukes were prepared as described previously, then fixed and processed for phalloidin staining.
These spines may facilitate the movement of juvenile flukes during their migration to the biliary system of the liver.
Transverse and longitudinal thick tissue sections of adult flukes (more than 12 weeks old) were fixed and processed for phalloidin staining as described above.
