The floating inflorescence remainders were decanted off, and the seeds were rinsed in distilled water.
The resin was then eluted a further 4 times, the eluate being decanted and retained each time.
The resulting liquid was then decanted with a pipette excluding any particulate matter that may have resulted from the reaction.
The supernatants were decanted and transferred to quartz cuvettes for absorption measurements.
The filtrate was allowed to settle for 15 min and the liquid decanted after this sedimentation.
Supernatant was then decanted to leave 2 cm sediment.
The sediment was stirred thoroughly to suspend all fine particles and decanted.
The bottles were then centrifuged at 600 g for 20 min and the supernatants decanted.
