For the immunolocalization study, muscles (normal or infected abdominal muscles) were fixed with 10% formalin, dehydrated and embedded in paraffin according to standard methods.
During acute illnesses, elderly patients can rapidly develop renal impairment especially if they are also dehydrated.
Another possible approach is heating of amino acids to bring about dehydrating condensation.
Usually the gel piece is partially dehydrated and the protease is added in the rehydration solution.
For this reason, only bacteria that had been dehydrated by freeze substitution through cold methanol are presented here.
However, the high temperatures would dehydrate the tissues and the possibility of workers being burnt led to this option being ruled out.
The frozen specimens are then freeze substituted, a process by which they are dehydrated in organic solvent while still frozen.
Other freshly-fixed specimens and some of the cryo-specimens were dehydrated in acetone and soaked in amyl acetate.
