Care must be taken not to pull too much clear glycerine into the needle.
As it emerged from the filter banks, it passed over glass plates covered in glycerine intended to trap the last remaining microbes.
Also, since glycerine is strongly hygroscopic, the prepared fluid must be kept sealed from the atmosphere as much as possible.
The purpose of the external fluidfilled vessel is to minimize the optical distortion due to the curved glycerine-wall-air interface.
When water was used instead of glycerine, much thinner layers were deposited.
We really have nothing in our equations to tell us whether the oil or the glycerine-water mixture will be on the wall.
All the nematodes recovered were fixed and preserved in 70% ethanol and 5% glycerine.
All nematode parasites were studied as wet mounts in a mixture of ethanol and glycerine.
