buffer Maksud & Definisi

Contoh buffer

• Pellets were resuspended in extraction buffer and protein was extracted twice as described above.

• In both yeast and nematodes, the ability of a gene to buffer the loss of its duplicate declines over time as their functional overlap decreases.

• Cells were washed with permeabilization buffer and then with staining buffer.

• The pellets were washed with 1 ml washing buffer and re-pelleted.

• Slides were removed from labelling buffer and excess buffer surrounding the tissue was wiped away.

• The pellet was resuspended in buffer and after 40 minutes this suspension was centrifuged as before.

• Upon centrifugation, the pellet, containing cellular matrix, was resuspended in high salt buffer to release cytoskeleton-associated components.

• Immunoprecipitated proteins were washed three times with 1 ml lysis buffer.