Thus, we focused on tracing labeled dendrites through serial sections using electron microscopy to determine their precise ultrastructural localization beneath the cone pedicle.
The peripheral cone pedicle thus can accommodate 84 horizontal cell terminals (two lateral elements per ribbon).
All of the contacts made by cone pedicles onto outer diffuse bipolar cells are also basal and symmetrically dense.
The quantification only included processes in the immediate postsynaptic region, either within the invagination proper or abutting the pedicle membrane in basal positions.
The density of outer diffuse bipolar cells, 23,327 cells mm 2, was only slightly smaller (88%) than the density of cone pedicles.
By contrast, the density of inner diffuse bipolar cells, 10,514 cells mm 2, was much smaller, just 40% of the density of cone pedicles.
The dendrites bore aggregates of terminals contacting the cone pedicles.
When a perforating vessel is found, it is dissected away from the surrounding muscle and traced to its origin from the vascular pedicle.