In this context, the storage conditions for larvae prior to inoculation are important.
I t appears that the initial rhizobia population per se is not a good indicator of inoculation response.
Consistently reproducible worm burdens were obtained without the need of immunosuppression or exsheathment of larvae prior to inoculation.
First, the high mortality induced by inoculation was independent of infection and time of inoculation of the beads.
In addition, six gerbils showed severe convulsion, with progressive emaciation and gerbils finally died from cachexia with neurological symptoms 2 weeks after inoculation.
Conversely, by increasing population, inoculation would bring economic benefits.
The possible contribution of a composting inoculum on decomposition and nutrient content was evaluated by subjecting the mixtures to inoculation.
This measure should avoid contact with artificially high shedding levels during the first days after inoculation.
