These results clearly indicate that caffeine treatment during prolonged maturation culture decreases the frequency of development to the blastocyst stage but not embryo quality.
The majority of caffeine-treated oocytes had a germinal vesicle with a nucleolus surrounded by ring- or horseshoe-shaped chromatin.
However, an association of caffeine use with breech birth was found.
However, no study of the effect of caffeine on the meiotic process of porcine oocytes has been performed.
The authors found that doses of caffeine higher than 450 g/ml in the culture medium inhibited oocyte maturation.
The initial fertility consultation: recommendations concerning cigarette smoking, body mass index, and alcohol and caffeine consumption.
Starvation is not the sole cause of caffeine-induced mortality, because several of the lines survive for longer on the drugged food than on agar.
Participants were permitted to use their typical amounts of tobacco and caffeine in order to avoid confounds associated with nicotine or caffeine withdrawal.
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