0 past simple and past participle of thaw --
1 to (cause to) change from a solid, frozen state to a liquid or soft one, because of an increase in temperature: --
2 to become friendlier or more relaxed: --
On the day of the assay, the samples were thawed and frozen quickly three times in liquid nitrogen to rupture cell membranes.
About 7 days before each individual test, worms were thawed and grown for at least 2 generations.
To ensure infectivity, the prepared vaccine must be used within 8 hours of thawing and, once thawed, should not be refrozen.
On the day of use, an aliquot was thawed and diluted in the culture medium to the desired working concentrations.
Immediately prior to being assayed, thawed saliva samples were centrifuged at 14,000 rpm for 7 min to remove particulate matter.
Using phase contrast microscopy, control slices showed more cell outgrowth compared to thawed slices.
Before use, they were thawed, added to the medium and subjected to sonication.
A fresh stock of virus was thawed immediately prior to each injection.
