The duration of this maternally derived immunity was determined experimentally from sera of unvaccinated children.
In total, 4943 (48n9 %) sera were from males and 5175 (51n1 %) from females.
All sera for which the result was equivocal were retested.
Saving tests by pooling sera - how great are the benefits ?
Sera has been systematically collected from mice and rats either harbouring a schistosome infection, or vaccinated with the isolated membrane preparation.
The sole exclusion criterion was sera collected from individuals with known immune deficiencies.
Blots were incubated overnight with the various test sera.
The wells were incubated for 2 h at room temperature with diluted human sera.