Both cumulus-free and cumulus-zona-free oocytes were rinsed thoroughly and kept in the medium for less than 30 min before use.
The seeds were then rinsed with sterile distilled water and agitated for 5 min in excess 70% ethanol.
Copepods were rinsed in filtered sea water and were directly added in tanks containing uninfected oysters.
Protoscoleces were aspirated from the melted cysts and rinsed several times with saline.
Following the priming treatments, seeds were thoroughly rinsed with water and dried to 5-6% water content.
Once completed, the object is rinsed with a solvent and then exposed in an ultraviolet oven that thoroughly cures the result.
To extract seeds, we thoroughly mixed each sample with water and rinsed it through sieves with decreasing mesh width (4, 2, 1 mm).
The incubation bottles were then removed, and the samples rinsed several times with washes of ' cold ' lagoon water.
