In refining our sugar a similar denaturing process 'is carried on.
The structure of the a-lactalbumin molten globule and denatured states are highly heterogeneous.
The pellet was dried, resuspended in urea dye, electrophoresed in a 10 % urea-acrylamide gel (denaturing and preparative gel) and transferred to nitrocellulose membrane.
Generally, the probe is purified from source and denatured into single strands and kept on ice until ready to be mixed with the labelling reaction.
When some structure is already partly formed, much smaller polymer motions are required to dock partially formed fragments than when the chain is completely denatured.
No differences could be detected, demonstrating that the protein was not irreversibly denatured by exposure to high temperature.
Given that the cosolvent denatured protein is relatively expanded, such secondary structure is likely to be lost quickly and refolding process is unaffected.
Drugs such as dapsone and sulphasalazine can cause non-immune haemolysis by oxidating and denaturing haemoglobin.
